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semidry western blot unit  (Bio-Rad)


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    Structured Review

    Bio-Rad semidry western blot unit
    Semidry Western Blot Unit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/semidry+blotting+unit/Western+Blot+Temperature+Sensitive+Reagents+Bag/pmc07195675-57-27-31
    Average 90 stars, based on 1 article reviews
    semidry western blot unit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Membrane:

    Article Title: Phenotypes of X-linked Charcot-Marie-Tooth disease and altered trafficking of mutant connexin 32 (GJB1).
    Article Snippet: .. Then, they were electrophoresed and transferred to an Immobilon polyvinylidene fluoride membrane (Millipore, Bedford, MA, USA), using a semidry blotting unit (Bio-RAD, Hercules, CA, USA) following Millipore’s recommendations. .. After being washed in blocking solution, the blots were incubated at 4°C overnight with a monoclonal antibody for Cx32 (M12.13) (Chemicon International Temecula, CA, USA) diluted to 1:100.

    Article Title: Altered trafficking and adhesion function of MPZ mutations and phenotypes of Charcot-Marie-Tooth disease 1B.
    Article Snippet: We previously reported familial cases characterized by Charcot-Marie-Tooth disease (CMT) phenotype with abnormal myelin foldings and MPZ Ile62Phe mutation.. To further clarify the molecular mechanisms in this family, we produced wild-type MPZ, Ile62Phe mutant and other mutations in the neighboring regions producing thin myelin sheaths (Ser63del, Ser63Cys and Ser63Phe) by site-specific mutagenesis and transfected these into rat pheochromocytoma cells (PC12).. We investigated the expression and aggregation properties of the MPZ protein through immunoblotting, immunohistochemical staining and adhesion assay.

    Incubation:

    Article Title: Altered trafficking and adhesion function of MPZ mutations and phenotypes of Charcot-Marie-Tooth disease 1B.
    Article Snippet: We previously reported familial cases characterized by Charcot-Marie-Tooth disease (CMT) phenotype with abnormal myelin foldings and MPZ Ile62Phe mutation.. To further clarify the molecular mechanisms in this family, we produced wild-type MPZ, Ile62Phe mutant and other mutations in the neighboring regions producing thin myelin sheaths (Ser63del, Ser63Cys and Ser63Phe) by site-specific mutagenesis and transfected these into rat pheochromocytoma cells (PC12).. We investigated the expression and aggregation properties of the MPZ protein through immunoblotting, immunohistochemical staining and adhesion assay.

    Nucleic Acid Electrophoresis:

    Article Title: Proinflammatory Cytokines Are Involved in the Initiation of the Abnormal Matrix Process in Pseudoexfoliation Syndrome/Glaucoma
    Article Snippet: Protein concentrations were determined by the Micro BCA Protein Assay kit (Thermo Scientific, Bonn, Germany). .. Ten micrograms of total protein was separated by SDS-polyacrylamide gel electrophoresis under reducing conditions and transferred onto nitrocellulose membranes (Hybond ECL; GE Healthcare, Munich, Germany) with a semidry blotting unit (Bio-Rad). .. Membranes were blocked with Superblock (Thermo Scientific) for 1 hour and incubated overnight at 4°C with mouse monoclonal antibodies against human p-STAT3 (clone 4; BD Biosciences, Heidelberg, Germany) or STAT3 (clone 84; BD Biosciences) diluted 1/500 or 1/2500 in Superblock, respectively.



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